Evaluation of Cytotoxic Activity Alkaloid Fractions of Zanthoxylum acanthopodium DC. Fruits

Dina Maya Syari; Rosidah Rosidah; Poppy Anjelisa Zaitun Hasibuan; Ginda Haro; Denny Satria

doi:10.3889/oamjms.2019.495

Authors

Dina Maya Syari Department of Pharmacology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia
Rosidah Rosidah Department of Pharmacology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia
Poppy Anjelisa Zaitun Hasibuan Department of Pharmacology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia
Ginda Haro Department of Biochemistry, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia
Denny Satria Department of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia

DOI:

https://doi.org/10.3889/oamjms.2019.495

Keywords:

Cytotoxic, Zanthoxylum acanthopodium DC., fruits, alkaloid, fraction

Abstract

AIM: This study was carried out to investigate cytotoxic activity towards T47D, 4T1, MCF-7, HeLa, and Raji cells of alkaloid fractions of Zanthoxylum acanthopodium DC. fruits. Zanthoxylum acanthopodium DC.

METHODS: The fruit was extracted by maceration. The ethanol extract was fractionated with liquid-liquid extraction using n-hexane, chloroform at pH 3,7, and 9 to obtained alkaloid fractions. Cytotoxic activity for fraction chloroform at pH 7 and 9 was determined with MTT assay.

RESULTS: The IC₅₀of fraction chloroform at pH 7 and 9 was (92.67 Â± 1.37; 71.87 Â± 1.04; 159.87 Â± 0.63; 123.39 Â± 0.81; and 103.09 Â± 0.58 Âµg/mLfor pH 7) and (451.29 Â± 25.48; 247.18 Â± 2.82; 318.46 Â± 5.40; 303.96 Â± 8.75; and 181.45 Â± 1.35 Âµg/mL for pH 9) respectively.

CONCLUSION: The results reveal that alkaloid fractions at pH 7 and 9 of Zanthoxylum acanthopodium DC. Fruits have cytotoxic activity. Our further study is to isolate and assesses anticancer activity from alkaloid compounds.