Cell Cycle Inhibition and Apoptotic Induction of Vernonia amygdalina Del. Leaves Extract on MCF-7 Cell Line

Authors

  • Poppy Anjelisa Zaitun Hasibuan Nanomedicine Center of Innovation, Universitas Sumatera Utara, Medan 20155, Indonesia; Departement of Pharmacology Pharmacy, Faculty of Pharmacy, Universitas Sumatera Utara, Medan 20155, Indonesia
  • Dian Fachrunisa

DOI:

https://doi.org/10.3889/oamjms.2019.509

Keywords:

Cytotoxicity, Vernonia amygdalina Del., MCF-7

Abstract

OBJECTIVE: The research aimed to determine the cytotoxic activity, cell cycle inhibition, and apoptosis induction of the ethyl acetate extract of the African leaves Vernonia amygdalina Del. on the MCF-7 cancer cells.

 

METHODS: The extraction of Vernonia amygdalina Del. leaves was done using the maceration method whereas the cytotoxic was performed using MTT assay. After that, the cell cycle testing and apoptosis induction were conducted using flow cytometry assay.

 

RESULTS: The IC50  values of n-hexane, ethyl acetate, and ethanol extract of Vernonia amygdalina Del. on the MCF-7 cancer cells were 206.211±0.99, 50,365±0.07, and 967.033 ± 2.68 µg/mL, respectively. The percentage of the cycle cell results in the G0-G1 phase in the cell control with 72.08% decreased in the treatment with ethyl acetate extract 1/2 IC50 with 62.58% and 1/5 IC50 with 44.72%. For the S and G2-M phase, the highest percentage was found in the ethyl acetate extract 1/5 IC50 treatment with 47.27% and 9.50% which were higher than the control cells with 23.26% and 5.90%.

 

CONCLUSION: Based on the results, the Vernonia amygdalina Del. extract provides chemopreventive agent as anti-cancer. Our future study will assess the mechanism of ethyl acetate fraction in inhibiting angiogenesis and metastatic in breast cancer.

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Published

2019-11-14

How to Cite

1.
Hasibuan PAZ, Fachrunisa D. Cell Cycle Inhibition and Apoptotic Induction of Vernonia amygdalina Del. Leaves Extract on MCF-7 Cell Line. Open Access Maced J Med Sci [Internet]. 2019 Nov. 14 [cited 2024 Mar. 28];7(22):3807-10. Available from: https://oamjms.eu/index.php/mjms/article/view/oamjms.2019.509

Issue

Section

Herbal Medicine in Pharmaceutical and Clinical Sciences

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