The Study of extract ethanol of Pirdot (Saurauria Vulcani Korth.) as immunostimulant in Rats (Rattus norvegicus)
DOI:
https://doi.org/10.3889/oamjms.2020.3876Keywords:
Saurauia vulcani Korth, Immunoglobulin G, Lysozyme activity, Renal histopathologyAbstract
BACKGROUND: Ethanolic extract of Pirdot leaves (Saurauria vulcani Korth) (EES) has bioactive compound to decrease glucose and increase insulin levels.
AIM: To know of effect of ethanolic extract of Pirdot leaves of immunoglobulin G and lysozyme activity in rats treated with Sheep Red Blood Cell (SRBC).
METHODS: Experimental design used in this research was Completely Randomized Design (CRD). There were 24 white rats to be classified into four groups: a control group with water (G0), a group treated with 0.1 ml sheep red blood cell (SRBC) (G1), a group treated with 500 mg/kg ethanol extract pirdot leaves (EES) (G2), and a group treated with 500 mg/KgBW ethanol Extract pirdot leaves (EES) + 0.1 ml SRBC (G3). Immunostimulant activity was evaluated by analyzingthe levels of immunoglobulin G with ELISA method, lysozyme with ABX Micros 60 and histophatology kidney with Hematoxylin-Eosin stainning.
RESULTS: The lysozyme activity in rats were treated with only SRBC () is higher and significantly different compared with a group of control (). Necrosis was not found in a group control () and rats treated EES 500 mg/kg ().There were no significant change in histophatology of kidney in rats treated EES 500 mg/kg. Disruption in bowman space with glomerulus was found in G1 (SRBC). Antigen induced disruption in G1 (SRBC) whereas giving extract ethanolic Pirdot leaves concurrently with antigen SRBC can protect kidney from disruption in G3.
CONCLUSIONS: A significantly effect of immunoglobulin G, lysozyme activity and histophatology kidney in rats after given by ethanolic extract of pirdot leaves.
Downloads
Metrics
Plum Analytics Artifact Widget Block
References
Jantan I, Ahmad W, Bukhari SN. Plant-derived immunomodulators: An insight on their preclinical evaluation and clinical trials. Front Plant Sci. 2015;6:1-18. https://doi. org/10.3389/fpls.2015.00655 PMid:28192772
Silitonga M, Ilyas S, Hutahaean S, Sipahutar H. Levels of apigenin and immunostimulatory activity of leaf extracts of bangunbangun (Plectranthus amboinicus Lour). Int J Biol. 2014;7(1):46-53. https://doi.org/10.5539/ijb.v7n1p46
Situmorang PC, Ilyas S. Review: Germinal cell apoptosis by herbal medicine. Asian J Pharm Clin Res. 2018;11(9):24-31. https://doi.org/10.22159/ajpcr.2018.v11i9.26400
Saghafi T, Taheri RA, Parkkila S, Emameh RZ. Phytochemicals as modulators of long non-coding rnas and inhibitors of cancer-related carbonic anhydrases. Int J Mol Sci. 2019;20(12):20122939. https://doi.org/10.3390/ ijms20122939 PMid:31208095
Lovena TN, Harahap U, Sitorus P, Satria D. Antioxidant Activity and α-Glucosidase Inhibition Effect of Water Extract of Saurauia vulcani Korth Leaves. Int J Pharm Biosci. 5 (3): 47-51.
Sinaga E, Ilyas S, Hutahaean S, Sitorus P. Immunostimulatory activity from pirdot leaves ethanolic extract (Saurauia vulcani Korth.) in rats (Rattus norvegicus). IOP Conf Ser Earth Environ Sci. 2019;305:012082. https://doi. org/10.1088/1755-1315/305/1/012082
Kumolosasi E, Ibrahim SN, Shukri SM, Ahmad W. Immunostimulant activity of standardised extracts of Mangifera indica leaf and curcuma domestica rhizome in mice. Trop J Pharm Res. 2018;17(1):77-84. https://doi.org/10.4314/tjpr. v17i1.12
Restuati M. Study of the extract activities of buas buas leaves (Premna pubescens) as immunostimulant on rats (Rattus novegicus). Am J Biosci. 2014;2(6):244. https://doi. org/10.11648/j.ajbio.20140206.19
Anderson HL, Brodsky IE, Mangalmurti NS. The evolving erythrocyte: Red blood cells as modulators of innate immunity. J Immunol. 2018;201(5):1343-51. https://doi. org/10.4049/jimmunol.1800565
Fatimah C. Universitas Sumatera Utara. I. Medan: Disertasi S3 Farmasi Fakultas Farmasi Unversitas Sumatera Utara; 2018. https://doi.org/10.15416/ijcp.2015.4.4.235
Hutapea A, Hutahaean S, Ilyas S. Influence of pirdot leaf (Saurauia vulcani Korth.) extract on the blood glucose rate and histologic description of the retina of male mice (Mus musculus strain ddw). Asian J Pharm Clin Res. 2018;11(11):389-92. https://doi.org/10.22159/ajpcr.2018.v11i11.28316
Taepongsorat L, Phadungkit M. Effects of Asparagus racemosus root extracts on serum lipid profiles, lipid peroxidation and superoxide dismutase in ovariectomized rat. Pharmacogn J. 2018;10(5):1036-41. https://doi.org/10.5530/pj.2018.5.175
Ilyas S, Hutahaean S, Nursal. Quantity and quality of guinea pig (Cavia porcellus) spermatozoa after administration of methanol extract of bitter melon (Momordica charantia) seed and depot medroxy progesterone acetate (DMPA). IOP Conf Ser Earth Environ Sci. 2018;130(1):012047. https://doi. org/10.1088/1755-1315/130/1/012047
Uluköy G, Kubilay A, Didinen BI, Kubilay A, Didinen B, Metin S, et al. Immunostimulant effects of geophyte plant extract on non-specific defence mechanisms of rainbow trout (Oncorhynchus mykiss). J Limnol Freshw Fish Res. 2018;4(1):36-41. https://doi. org/10.17216/limnofish.376404
Chinnappan SM, George A, Thaggikuppe P, Choudhary Y, Choudhary VK, Ramani Y, et al. Nephroprotective effect of herbal extract Eurycoma longifolia on paracetamol-induced nephrotoxicity in rats. Evid Based Complement Altern Med. 2019;2019:4916519. https://doi.org/10.1155/2019/4916519
Hanafiah OA, Hanafiah DS, Bayu ES, et al. Quantity differences of secondary metabolites (Saponins, tannins, and flavonoids) from binahong plant extract (Anredera cordifolia (Ten.) Steenis) treated and untreated with colchicines that play a role in wound healing. World J Dent. 2017;8(4):296-9. https://doi.org/10.5005/ jp-journals-10015-1453
Hasibuan R, Ilyas S, Hanum S. Effect of leaf extract haramonting (Rhodomyrtus tomentosa) to lower blood sugar levels in mice induced by alloxan. Int J PharmTech Res. 2015;8(6):284-91.
Masyithah C, Hadisahputra S, Ilyas S. Combinational effects of ethylacetate extract of Zanthoxylum acanthopodium DC. With doxorubicinon MCF7 breast cancer cells. Int J PharmTech Res. 2015;7(4):974-4304.
Ilyas S. Histological changes in the testis if the guinea pig during administration of methanol extract of bitter melon (Momordica charantia L.) seed and DMPA. J Phys Conf Ser. 2018;1116(5):052031. https://doi. org/10.1088/1742-6596/1116/5/052031
Sabri E, Ilyas S, Prasetiawan E. The effect of n-hexane extract of andaliman (Zanthoxylum acanthopodium DC.) fruit in the liver tissues of mice during post implantation of pregnancy. J Phys Conf Ser. 2018;1116(5):1-6. https://doi. org/10.1088/1742-6596/1116/5/052056
Ilyas S. Effect of methanolic Momordica charantia seed extract and depot medroxyprogesterone acetate (DMPA) to quantity and quality of rat sperm. Int J PharmTech Res. 2014;6(6):974-4304.
Sari MI, Ilyas S, Widyawati T, Antika MA. Effect of Lawsonia innermis (linn) leaves ethanolic extract on blood glucose and malondialdehyde level in alloxan-induced diabetic rats. IOP Conf Ser Earth Environ Sci. 2018;130(1):012034. https://doi. org/10.1088/1755-1315/130/1/012034
Hutahaean S, Banjarnahor RD, Darsini P, Ilyas S, Sabri E. The effect of Saurauia vulcani Korth. leaves extract on penile corpus cavernosa microstructure and the quality of sperm in alloxan-induced diabetic mice. J Phys Conf Ser. 2018;1116(5):052030. https://doi.org/10.1088/1742-6596/1116/5/052030
Ilyas S, Hutahaean S, Nursal. Apoptosis overview of cerebellum Purkinje cell in mice (Mus musculus L.) after exposure to methanol extract of the seeds of bitter melon (Momordica charantia) and DMPA. Int J PharmTech Res. 2016;9(9):444- 449. https://doi.org/10.22159/ajpcr.2018.v11i12.27920
Ilyas S, Hutahaean S, Nursal. Transforming growth factor beta of testis germinal cell in Guinea pig (Cavia porcellus) after exposure to methanol extract of the seeds of bitter melon (Momordica charantia) and depot medroxyprogesterone acetate. Asian J Pharm Clin Res. 2018;11(12):149-154. https:// doi.org/10.22159/ajpcr.2018.v11i12.27920
Ilyas S, Tanjung RS, Thahira D, Wulandari J. Effectiveness of administration haramounting leaf (Rhodomyrtus tomentosa) as antioxidant in preventing the damage pancreas mice (Mus musculus L.) after exposure of electric cigarette smoke. J Phys Conf Ser. 2018;1116(5):052084. https://doi. org/10.1088/1742-6596/1116/5/052084
Lee HJ, Jeong SE, Lee S, Kim S, Han H, Jeon CO. Effects of cosmetics on the skin microbiome of facial cheeks with different hydration levels. Microbiologyopen. 2018;7(2):e00557. https:// doi.org/10.1002/mbo3.557 PMid:29193830
Masfria, Hap UH, Nasution MP, Ilyas S. Cytotoxic activity, proliferation inhibition and apoptosis induction of Rhaphidophora pinnata (L.F.) schott chloroform fraction to MCF-7 cell line. Int J PharmTech Res. 2014;6(4):1327-33.
Kane SN, Mishra A, Dutta AK. Preface: International conference on recent trends in physics (ICRTP 2016). J Phys Conf Ser. 2016;755(1):011001. https://doi. org/10.1088/1742-6596/534/1/011001
Kpolovie P. Statistical Analysis with Spss for Research. United Kingdom: ECRTD Publication; 2017.
Widowati W, Fauziah N, Herdiman H, Afni M, Afifah E, Kusuma S, et al. Antioxidant and Anti Aging Assays of Oryza sativa extracts, vanillin and coumaric acid. J Nat Remedies. 2016;16(3):88. https://doi.org/10.18311/jnr/2016/7220
Zain SN, Omar WA. Antioxidant activity, total phenolic content and total flavonoid content of water and methanol extracts of phyllanthus species from Malaysia. Pharmacogn J. 2018;10(4):677-81. https://doi.org/10.5530/pj.2018.4.111
Gulce Iz S, Saglam Metiner P, Kimiz I, Kayali C, Deliloglu Gurhan SI. Polyclonal antibody production against hapten-structured KDN molecule by using different adjuvants alternative to freund’s adjuvant. Eur J Ther. 2018;24(2):106-11. https://doi. org/10.5152/eurjther.2018.400
Delivery ND, Snedds S, Wijiyanto B, Sitorus MA, Listianingrum RD, Dea AC, et al. Formulasi sediaan nano herbal tempuyung (Sonchus arvensis L.) dalam bentuk self nano-emulsifying drug delivery system (SNEDDS). 2016;3(1):50-3. https://doi.org/10.29208/jsfk.2016.3.1.99
Ilyas S, Lestari SW, Moeloek N, Asmarinah, Siregar NC. Induction of rat germ cell apoptosis by testosterone undecanoate and depot medroxyprogesterone acetate and correlation of apoptotic cells with sperm concentration. Acta Med Indones. 2013;45(1):32-7. https://doi.org/10.13181/mji.v17i3.313 PMid:23585406
Herwanto RY, Bashiruddin J, Ilyas S, Lubis MN. Correlation of noise intensity to heat shock response with ultrastructure region of Rattus norvegicus cochlea. 2015;7(1):80-4.
Yusuf F, Ilyas S, Damanik HA, Fatchiyah F. Microbiota composition, HSP70 and caspase-3 expression as marker for colorectal cancer patients in aceh, Indonesia. Acta Med Indones. 2016;48(4):289-99. PMid:28143990
Moeloek N, Asmarinah, Siregar NC, Ilyas S. Testosterone undecanoate and depo medroxyprogesterone acetate induced azoospermia through increased expression of spermatogenic cell caspase 3. Med J Indones. 2008;17(3):313. https://doi. org/10.13181/mji.v17i3.313
Ilyas S, Hutahaean S, Irianti DE. Transforming growth factor expression (TGF-β) correlate with serum level of malondialdehyde (MDA) after EVOO administration in preclinical rat models of preeclampsia. IOP Conf Ser Earth Environ Sci. 2018;130(1):012048. https://doi. org/10.1088/1755-1315/130/1/012048
Satria D, Silalahi J, Haro G, Ilyas S, Hsb PA. Antioxidant and antiproliferative activities of an ethylacetate fraction of picria fel-terrae Lour. herbs. Asian Pacific J Cancer Prev. 2017;18(2):399- 403. https://doi.org/10.5220/0008359701900193
Situmorang PC, Ilyas S. Study of preeclampsia in placenta, kidney, and hepatic diseases. Asian J Pharm Clin Res. 2018;11(11):21-8. https://doi.org/10.22159/ajpcr.2018. v11i11.27540
Situmorang PC, Ilyas S. Description of kidney histology of Mus musculus after giving nano herbal Rhodomyrtus tomentosa (Haramonting). Int J Ecophysiol. 2019;1(1):26-33. https://doi. org/10.22159/ajpcr.2018.v11i11.29042
Downloads
Published
How to Cite
License
Copyright (c) 2020 Erlintan Sinaga, Syafruddin Ilyas, Salomo Hutahaean, Panal Sitorus (Author)
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
http://creativecommons.org/licenses/by-nc/4.0
