Cyclin D1, Caspase 9 and P53 Expressions in T47D Cell Lines after Treatment of Plectranthus amboinicus, (Lour.) Spreng. Leaves Ethanolic Extract Nanoparticles

Authors

  • Poppy Anjelisa Zaitun Hasibuan Nanomedicine Center of Innovation, Universitas Sumatera Utara, Medan 20155, Indonesia; Departement of Pharmacology Pharmacy, Faculty of Pharmacy, Universitas Sumatera Utara, Medan 20155, Indonesia
  • Sumaiyah Sumaiyah Departement of Technology Pharmacy, Faculty of Pharmacy, Universitas Sumatera Utara, Medan 20155, Indonesia

DOI:

https://doi.org/10.3889/oamjms.2019.504

Keywords:

Plectranthus ambonicus, nanoparticle, cyclin D1, caspase-9, p53

Abstract

BACKGROUND: The application of nanotechnology is aimed to enhance the capability of the chemical compounds of Plectranthus amboinicus (Lour.) Spreng. leaves activity. The contact area of the particle surface becomes larger in nano size, which can increase the amount of the isolated active substance. The nanoparticles of this extract exhibited the most effective impact in decreasing the cell number of T47D by induction of apoptosis and arresting the cell cycle.

AIM: To evaluate the expressions of cyclin D1, caspase-9 and p53 in T47D cell lines treated by Plectranthus amboinicus, (Lour.) Spreng. Leaves Ethanolic Extract Nanoparticles (PAEEN).

METHODS: The inhibition of cyclin D1 was done by flow cytometry assay. The expression of cyclin D1, caspase-9 and p53 were observed by immunocytochemistry assay.

RESULTS: Plectranthus amboinicus, (Lour.) Spreng. Leaves Ethanolic Extract Nanoparticles (PAEEN) on concentration IC50 (89.2 µg/mL) inhibited the expression of cyclin D1 around 5.59%. Further, the immunocytochemistry assay indicated that there was an inhibition of cyclin D1, upregulation of caspase-9 and restoration of p53, indicated by their expression.

CONCLUSIONS: The Plectranthus amboinicus, (Lour.) Spreng. Leaves Ethanolic Extract Nanoparticles could be an effective anticancer by improving its downregulation on cyclinD1, upregulation caspase-9 and p53.

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Published

2019-11-14

How to Cite

1.
Hasibuan PAZ, Sumaiyah S. Cyclin D1, Caspase 9 and P53 Expressions in T47D Cell Lines after Treatment of Plectranthus amboinicus, (Lour.) Spreng. Leaves Ethanolic Extract Nanoparticles. Open Access Maced J Med Sci [Internet]. 2019 Nov. 14 [cited 2024 Mar. 29];7(22):3786-9. Available from: https://oamjms.eu/index.php/mjms/article/view/oamjms.2019.504

Issue

Section

Herbal Medicine in Pharmaceutical and Clinical Sciences

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